Figure A5.

Lack of the 1IFNR in MutuDC lines has a positive or negative impact on mRNA expression of several genes upon stimulation. WT (n = 2) or 1IFNR (n = 2) MutuDC lines were stimulated for 20 h with different combinations of CpG, PolyIC, IFNγ, and aCD40 as indicated and described in Figure 7B, and analyzed by Nanostring technology. Figure 7C provides a summary table for the positive or negative effects of the 1IFNR on mRNA expression of several genes upon stimulation. Here, representative examples of positive (A) or negative (B) effects (four per group) are shown. Upon stimulation, the decrease in mRNA expression due to lack of 1IFNR (“positive” effect of 1IFNR) was 2- to 13-fold for il10, 3- to 17-fold for Ifnβ, 5- to 20-fold for Gmcsf and 5- to 36-fold for Serpinb1a. On the other hand the lack of 1IFNR resulted in an increase in mRNA expression (“negative” effect of 1IFNR) that was 4- to 10-fold for Cxcl9, 3- to 7-fold increase for Ccl3, 3 to 18-fold for B7dc and 2- to 13-fold for Tnfa.