Figure A6.

MutuDC lines keep their ability to upregulate activation markers upon stimulation even at high passage numbers. (A) Cells with low (33, open bars) or high (98, black bars) passage number were stimulated for 20 h with different combinations of CpG, PolyIC, IFN-g, and aCD40 as indicated. Analysis of CD40 expression by flow cytometry is shown (similar results were obtained for CD80, CD86, and MHC-II, data not shown). (B) MutuDC lines at p33 or p98 were stimulated as indicated [similar to (A)] and were analyzed for IL-12 p40 and p70 secretion in the cell culture supernatants.