Table II.
Characteristics of A. maculatum mitochondria isolated from appendix tissue of inflorescences in the δ-stage with an appendix temperature of >26°C or <24°C
| Assay Condition | Appendix Temperature
>26°C
|
Appendix Temperature <24°C
|
||
|---|---|---|---|---|
| O2 uptake | UQ10 reduction | O2 uptake | UQ10 reduction | |
| nmol min−1 mg−1 protein | % of total | nmol min−1 mg−1 protein | % of total | |
| Succinate | ||||
| + ADP | 197 ± 22 | nda | 185 ± 24 | nd |
| + ADP + CN | 199 ± 20 | nd | 186 ± 20 | nd |
| + CN + pyruvate | 209 ± 19 | 48 | 193 ± 15 | 51 |
| + ADP + OG | 51 ± 21 | 45 | 41 ± 15 | 46 |
| NADH | ||||
| + ADP | 777 ± 35 | nd | 782 ± 56 | nd |
| + ADP + CN | 776 ± 34 | nd | 783 ± 55 | nd |
| + CN + pyruvate | 931 ± 34 | 93 | 914 ± 32 | 89 |
Succinate (20 mm) or NADH (2 mm) were given as respiratory substrates in the absence or presence of 0.15 mm ADP. CN-resistant respiration (+ 0.1 mm CN) was measured in the absence or presence of 5 mm pyruvate. Cyt pathway activity was measured in the presence of 2 μm octyl gallate (OG). Data are mean values ± sd of triplicate (O2 uptake) or single (UQ10 reduction) measurements of one mitochondrial isolation.
nd, Not determined.