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Gel filtration of III-24. (a) Sample TCEP-reduced after 18 h at 4 °C; (b) 2 h at 4 °C; (c) 14 d at 4 °C (14 d at −20 °C is similar). (d) After freeze thawing 10× during a 14 d period at −20 °C; (e) 80 d at −20 °C; (f) 37 month at 4 °C.
m: monomer – Stokes’ Radius (R) = 1.3 nm; H: 1 triple helix, R = 1.9 nm; 2H: 2 triple helices, R = 2.7 nm; 4H: ∼3–5 triple helices, R = 4.0 nm; A: small aggregate R = 5.7 nm; s: solvent (right-most peak).
