Analysis of sugar products generated by protein
extracts of transformed protoplasts after incubation with different Suc
concentrations. Sugar products were analyzed by HPLC after a 24-h
incubation with 20 mm (A, B, and C) or 100 mm
(D and E) Suc at 27°C. A and D, Protein extract from protoplasts
transformed with AcN2 under control of the 35S promoter; B and E,
protein extract from protoplasts transformed AcT1 under control of the
35S promoter; C, protein extract from protoplasts transformed with
empty vector; and F, sugar extract from chicory root. Peaks in the HPLC
chromatograms: T, trehalose (internal standard); G, Glc; F, Fru; S, Suc
(G1–2F); 1-K, 1-kestose (G1–2F1–2F); DP4–6, inulin fructan with a
degree of polymerization of 4 to 6.