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. 1998 Aug;117(4):1507–1513. doi: 10.1104/pp.117.4.1507

Figure 4.

Figure 4

Analysis of sugar products generated by either single or combined incubation of protein extracts from transformed protoplasts with different substrates. Sugar products were analyzed by HPLC after 24 h of incubation at 27°C. A, Combined incubation of protein extracts from protoplasts transformed with onion 1-SST and 6G-FFT with 100 mm Suc; B, single incubation of protein extract from protoplasts transformed with onion 6G-FFT with 20 mm Suc and 20 mm 1-kestose; C, sugar products from onion bulb; D, combined incubation of protein extracts from protoplasts transformed with onion 1-SST and barley 6-SFT with 100 mm Suc; E, single incubation of protein extract from protoplasts transformed with barley 6-SFT with 100 mm Suc; and F, single incubation of protein extract from protoplasts transformed with onion 1-SST with 100 mm Suc. Peaks in the HPLC chromatograms: T, trehalose (internal standard); G, Glc; F, Fru; S, Suc (G1–2F); 1-K, 1-kestose (G1–2F1–2F); N-K, neokestose (F2–6G1–2F); N, nystose (G1–2F1–2F1–2F); 6-K, 6-kestose (G1–2F6–2F); BiF, bifurcose (G1–2F1[6–2F]1–2F).