Fig 2.

The adult intestinal stem cell marker gene LGR5 is upregulated at the climax of intestinal metamorphosis. Cross-sections of the intestine at premetamorphic stage 54 (A, A'), metamorphic climax stage 62 (B, B'), and the end of metamorphosis (stage 66) (C, C') were analyzed by in situ hybridization for LGR5 expression. Arrows indicate the cells expressing LGR5 (A'-C'). Higher magnification of boxed areas in (A)-(C) are shown in (A')-(C'). AE: adult epithelial progenitor/stem cells, CT: connective tissue, LE: larval epithelial cell, Lu: lumen, M: muscle layer, Ty: typhlosole. Scale bars are 100 µm (A-C) and 20 µm (A'-C'), respectively. Based on ¹⁴.