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. 2012 Oct 8;109(43):17591–17596. doi: 10.1073/pnas.1209411109

Fig. 1.

Fig. 1.

Gluocorticoids differentially regulate β-arrestin-1 and β-arrestin-2 gene expression. (A) A549 cells were treated with 100 nM Dex for the indicated times, and β-arrestin-1 and β-arrestin-2 mRNA were analyzed by RT-PCR. (B) A549 cells were treated with 100 nM Dex for the indicated times, and β-arrestin-1 and β-arrestin-2 protein were evaluated by immunoblot analysis. Shown are the quantitation of β-arrestin-1 and β-arrestin-2 normalized to β-actin and a representative immunoblot. (C) U2OS cells stably expressing GRs were treated with 10 nM Dex for the indicated times, and β-arrestin-1 and β-arrestin-2 were evaluated by RT-PCR and immunoblot analysis. Data represent mean ± SD from three or four independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.