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Thrashing-defective phenotype of tau V337M animals was rescued by the ogt-1(ok430) mutation. (A) Mean number of thrashing movements ± SEM. (B) Immunoblot of tau protein in WT and O-GlcNAc cycling mutant animals. Protein extract was analyzed by immunoblotting with the following antibodies: phosphorylation-independent tau (pan-tau), pS409, pS396, and pS214 for specific phosphorylated serine residues; and AT-8 for pS202/pT205. Data are shown as mean ± SD.
