Fig. 4.

MLL2 regulates the expression of retinoic acid target genes. (A) Reduced expression of ASB2 (a retinoic acid response gene) in MLL2^−/− cells, as determined by RT-qPCR. (B) Attenuated ASB2 gene expression in response to retinoic acid treatment in MLL2^−/− cells. Four MLL2^−/− lines derived from two different MLL2^+/− progenitor lines were treated with DMSO or with 1 μM retinoic acid for 48 h. Quantitative RT-PCR was performed to determine the relative expression level of ASB2 genes. Error bars stand for standard deviation from three independent experiments.