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. 2012 Sep 10;109(43):17388–17393. doi: 10.1073/pnas.1208642109

Fig. 4.

Fig. 4.

Anti-CC is essential for Myo10’s function. (A and B) Representative images of HeLa cells transfected with various GFP-tagged Myo10 constructs, actin filament with phalloidin (red), and nuclei with DAPI (blue): (A1), Motor-CC(1-934)(WT); (A2), Motor-SAH; (A3), Motor-CC(1-934)(L893Q); (A4), Motor-CC(1-934)(K904A); (A5), Motor-SAH-GCN4; (B1), Motor-CC(WT)-Tail; (B2), Motor-CC(L893Q)-Tail; (B3), Motor-CC(K904A)-Tail; (B4), Motor-SAH-GCN4-Tail. (Scale bar, 5 μm.) (C) Quantitative analysis of GFP puncta number per cell at the tip of filopodia (C1) and filopodial number (C2) (with length >0.5 μm). Values are means ± SD from three independent experiments with 10 cells per experiment, using unpaired t test, *P < 0.05, *P < 0.01, ***P < 0.001. Also refer to Fig. S7 for data in COS7 cells.