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. 2012 Oct 8;109(43):E2949–E2955. doi: 10.1073/pnas.1207718109

Fig. 3.

Fig. 3.

BARD increases DNA damage signaling. Effect of BARD on appearance and disappearance of DNA damage-associated repairosome foci of CtIP (A) and 53BP1 (B) after 5-Gy doses of IR. (C) Rad51-positive cells with vehicle or BARD 4 h after 5-Gy doses of IR. For each point, 200 cells were counted, and the mean of three experiments is given in the figures. (D) Western blot of Rad51 and BRCA1 levels in the nuclear fraction of HCECs 30, 60, and 120 min after 5-Gy doses of IR with or without BARD pretreatment. 53BP1 foci-positive cells (E) and nuclear localization of Rad51 (F) in Nrf2 knockdown cell line 60 min after 5-Gy doses of IR. *P < 0.05 (compared with vehicle control) in the unpaired Student t test. shCtrl, short hairpin control; shNrf2, short hairpin Nrf2.