Table I.
KRIs and relative intensities of characteristic diagnostic ions as determined by GC-MS-SIM for permethylated authentic CK standards and putative CK purified from tissues of developing chickpea seeds
| Compound | KRI | Constituent Ions | ||
|---|---|---|---|---|
| m/z (relative abundance) | ||||
| [9R]Z | ||||
| [2H5]trans-[9R]Z | 3084 | 221a(100) | 395 (48) | 426 (2) |
| Authentic trans-[9R]Z | 3089 | 216 (100) | 390 (52) | 421 (2) |
| Putative trans-[9R]Z | 3089 | 216a(100) | 390 (50) | 421 (3) |
| Putative trans-[9R]Z from hydrolyzed trans-[9R-MP]Z | 3089 | 216a(100) | 390 (62) | |
| Authentic cis-[9R]Z | 3065 | 216 (100) | 390 (54) | 421 (2) |
| Putative cis-[9R]ZR | 3065 | 216a(100) | 390 (46) | 421 (2) |
| Putative cis-[9R]Z from hydrolyzed cis-[9R-MP]Z | 3065 | 216a(100) | 390 (45) | 421 (3) |
| [9R]DHZ | ||||
| [2H3][9R]DHZ | 2997 | 163a(100) | 177 (64) | 253 (78) |
| Authentic [9R]DHZ | 2999 | 162 (100) | 176 (65) | 250 (74) |
| Putative [9R]DHZ | 2998 | 162a(100) | 176 (47) | 250 (32) |
| Putative [9R]DHZ from hydrolyzed [9R-MP]DHZ | 2998 | 162 (100) | 176 (35) | |
| Z | ||||
| [2H5]trans-Z | 2299 | 235a(100) | 188 (18) | 266 (4) |
| Authentic trans-Z | 2304 | 230 (100) | 188 (24) | 261 (5) |
| Putative trans-Z | 2304 | 230a(100) | ||
| Authentic cis-Z | 2280 | 230 (100) | 188 (25) | 261 (5) |
| Putative cis-Z | 2280 | 230a(100) | 188 (25) | 261 (5) |
| DHZ | ||||
| [2H3]DHZ | 2223 | 177a(100) | 191 (25) | 235 (12) |
| Authentic DHZ | 2225 | 176 (100) | 190 (30) | 232 (22) |
| Putative DHZ | 2224 | 176a(100) | 190 (38) | 232 (28) |
| iP | ||||
| [2H6]iP | 2090 | 188 (100) | 237a(42) | 219 (47) |
| Authentic iP | 2096 | 188 (100) | 231 (36) | 216 (44) |
| Putative iP | 2096 | 188 (100) | 231a(32) | |
| [9R]iP | ||||
| [2H6][9R]iP | 2876 | 174 (88) | 397a(100) | 205 (44) |
| Authentic [9R]iP | 2882 | 174 (86) | 391 (100) | 202 (78) |
| Putative [9R]iP | 2882 | 174 (75) | 391a(100) | |
| Putative [9R]iP from hydrolyzed [9R-MP]iP | No detection made except for internal standard | |||
a
Ions used in quantification.