Table II.
Concentration of CK identified from components of developing chickpea seeds as quantified by isotope dilution assay using GC-MS-SIM
| Compound | CK Concentration
|
|||||
|---|---|---|---|---|---|---|
| Poda1 DAPS | Embryo 14 DAPS | Seed Coat 14 DAPS | Endosperm 14 DAPS | Cotyledon
|
||
| 30 DAPS | 40 DAPS | |||||
| pmol g−1 dry wt | ||||||
| cis-[9R-MP]Z | 3405 ± 732 | 6106 | 2133 ± 1216 | 12,381 ± 2,434 | 193 ± 46 | 190 ± 20 |
| trans-[9R-MP]Z | 256 ± 129 | ndb | 101 ± 52 | 1,993 ± 667 | 15 ± 4 | nd |
| [9R-MP]DHZ | 117 ± 117 | nd | 166 ± 166 | nd | nd | nd |
| cis-[9R]Z | 403 ± 51 | 1572 | 98 ± 21 | 3,149 ± 1,082 | 152 ± 38 | 37 ± 6 |
| trans-[9R]Z | 153 ± 66 | nd | 39 ± 2 | nd | 3 ± 1 | 8 ± 5 |
| [9R]DHZ | 299 ± 50 | 2414 | 81 ± 32 | 534 ± 77 | nd | 8 ± 7 |
| cis-Z | 80 ± 40 | nd | 53 ± 43 | 130 ± 130 | 12 ± 3 | nd |
| trans-Z | 42 ± 42 | 231 | 24 ± 2 | nd | 4 ± 1 | 15 ± 4 |
| DHZ | 1 ± 1 | nd | 24 ± 20 | nd | 6 ± 2 | 6 ± 5 |
| [9R]iP | nd | nd | nd | nd | 6 ± 1 | 7 ± 5 |
| iP | nd | nd | nd | nd | 9 ± 5 | 2 ± 2 |
| [9R-MP]iP | nd | nd | nd | nd | nd | nd |
Data are means ± se (n = 3–6).
a
Fertilized ovary.
b
nd, Not detected.