Fig. 1.
The Pikfyveβ-geo/β-geo mutant has low levels of Pikfyve protein. (A) Schematic of the Pikfyve coding region indicating position of the β-Geo insert, the 5′ and 3′ regions with primers spanning exons 2 and 3 and exons 41 and 42 used for quantitative RT-PCR (green arrows), the shRNA target site (red arrow), and peptide (asterisk) used to produce the mouse monoclonal antibody. (B) Western blots show that Pikfyveβ-geo/β-geo fibroblasts have reduced Pikfyve protein but no difference in Vac14 and Fig4 protein levels.