Fig 3.

Microscopic pneumonia scores and IAV antigen scores at 5 days postinfection. Tissues were collected from pigs vaccinated intranasally with Ad5-empty (white bars) or Ad5-HA (black bars) 42 days prior to challenge or intramuscularly with kaCA (gray bars) 42 and 21 days prior to challenge. Pigs were challenged intranasally with A/CA/04/09 (CA09), A/SW/MN/2011/08 (MN08), or PBS (NC). Trachea (A) and lung (B) histopathology scores are shown for hematoxylin-and-eosin-stained formalin-fixed tissues collected 5 days following challenge with CA09 or MN08. (C) Lung IAV antigen scores identified using an anti-NP (HB65) antibody on formalin-fixed tissue 5 days after infection with CA09 or MN08 as described in Materials and Methods. Results are reported as means ± SEM, and statistical differences between nonvaccinated and vaccinated groups challenged with the same virus are indicated with connecting bars and asterisks (P ≤ 0.05).