Table 5.
Anti-PA IgG and TNA ED50 levels associated with 80% probability of survival in AVA-vaccinated animals at different study time points up to study month 52a
| Time postvaccination (mo) | Total anti-PA IgG (μg/ml) (95% CI) | Intercept (P value) | Slope (P value) | H-L goodness-of-fit test P valueb | Area under ROC curve (%) | TNA (ED50) (95% CI) | Intercept (P value) | Slope (P value) | H-L goodness-of-fit test P valueb | Area under ROC curve (%) |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 6.4 (0, >10max) | 0.51 (0.1595) | 1.08 (0.0720) | 0.715 | 59.9 | 41 (20, >10max) | −0.96 (0.3409) | 1.46 (0.0466*) | 0.715 | 61.7 |
| 2 | 30.4 (12.5, 170.3) | −0.37 (0.4010) | 1.18 (0.0005*) | 0.266 | 73.5 | 338 (124, 2,443) | −1.26 (0.0677) | 1.05 (0.0007*) | 0.042 | 73.0 |
| 3 | 4.8 (1.5, 37.1) | −0.02 (0.9766) | 2.06 (0.0240*) | 0.930 | 73.9 | |||||
| 4 | 4.1 (1.4, 24.4) | 0.26 (0.6284) | 1.85 (0.0249*) | 0.0279 | 72.7 | 36 (14, 170) | −1.27 (0.2282) | 1.71 (0.0129*) | 0.011 | 70.7 |
| 5 | 3.5 (0.6, 149.2) | 0.36 (0.5071) | 1.87 (0.0425*) | 0.255 | 69.3 | 30 (5, 249) | −0.89 (0.4076) | 1.54 (0.0301*) | 0.759 | 69.2 |
| 6 | 8.6 (4.7, 1,336.3) | −0.24 (0.6493) | 2.62 (0.0161*) | 0.114 | 69.0 | 42 (25, 205) | −1.94 (0.0633) | 2.05 (0.0054*) | 0.567 | 68.7 |
| 6.5 | 5,712 (1,621, >10max) | −3.31 (0.0970) | 1.25 (0.0446*) | 0.483 | 70.9 | |||||
| 7 | 97.3 (49.4, 329.7) | −1.72 (0.0256*) | 1.56 (0.0003*) | 0.241 | 75.4 | 1,243 (621, 4,479) | −3.31 (0.0077*) | 1.52 (0.0005*) | 0.617 | 74.5 |
| 8 | 25.0 (12.8, 81.1) | −0.84 (0.1002) | 1.59 (0.0001*) | 0.175 | 76.2 | 312 (143, 1,258) | −2.02 (0.0160*) | 1.36 (0.0002*) | 0.067 | 75.2 |
| 9 | 14.1 (8.0, 38.0) | −0.83 (0.0797) | 1.93 (0.0001*) | 0.692 | 78.0 | 143 (70, 493) | −1.91 (0.0104*) | 1.53 (0.0001*) | 0.239 | 76.9 |
| 10 | 11.6 (6.5, 34.6) | −0.61 (0.1737) | 1.87 (0.0002*) | 0.440 | 76.3 | 124 (64, 422) | −2.00 (0.0090*) | 1.62 (0.0001*) | 0.187 | 76.6 |
| 11 | 7.8 (5.0, 18.8) | −0.78 (0.0965) | 2.43 (0.0001*) | 0.920 | 77.6 | 106 (55, 368) | −1.91 (0.0138*) | 1.63 (0.0002*) | 0.720 | 75.2 |
| 11.75 | 7.0 (4.6, 16.4) | −0.79 (0.0932) | 2.57 (0.0002*) | 0.68 | 77.5 | 92 (48, 345) | −1.80 (0.0266*) | 1.62 (0.0005*) | 0.192 | 72.7 |
| 12.5 | 5.4 (2.5, 15.2) | −1.23 (0.2358) | 3.58 (0.0086*) | 0.830 | 85.9 | 56 (4, 295) | −1.74 (0.2399) | 1.79 (0.0209*) | 0.705 | 79.5 |
| 15.5 | 5.6 (2.9, 14.6) | −0.98 (0.2602) | 3.18 (0.0060*) | 0.710 | 80.8 | 60 (23, 207) | −2.63 (0.0696) | 2.25 (0.0055*) | 0.777 | 80.7 |
| 18 | 5.0 (2.6, 14.8) | −0.86 (0.3249) | 3.22 (0.0101*) | 0.797 | 81.6 | 49 (23, 149) | −3.15 (0.0423*) | 2.68 (0.0042*) | 0.959 | 82.6 |
| 21 | 4.2 (2.5, 11.1) | −1.08 (0.2522) | 3.98 (0.0110*) | 0.986 | 81.9 | 43 (24, 118) | −4.05 (0.0189*) | 3.33 (0.0028*) | 0.963 | 86.1 |
| 24 | 3.9 (2.2, 11.1) | −0.87 (0.3467) | 3.84 (0.0154*) | 0.971 | 80.6 | 41 (22, 125) | −3.56 (0.0343*) | 3.07 (0.0050*) | 0.9,737 | 83.4 |
| 27.25 | 3.9 (2.8, 72.3) | −1.96 (0.1323) | 5.63 (0.0304*) | 0.940 | 78.2 | 54 (26, 1,291) | −3.46 (0.0596) | 2.80 (0.0189*) | 0.676 | 78.8 |
| 30 | 3.9 (2.9, 35.9) | −2.68 (0.0775) | 6.90 (0.0261*) | 0.591 | 83.3 | 65 (28, 1,644) | −2.99 (0.0817) | 2.42 (0.0201*) | 0.645 | 77.2 |
| 33 | 3.9 (0, >10max) | −1.41 (0.3228) | 4.70 (0.0870) | 0.800 | 78.6 | 82 (34, 1,194) | −5.32 (0.0661) | 3.50 (0.0263*) | 0.957 | 84.1 |
| 36 | 3.8 (0, >10max) | −1.63 (0.2652) | 5.22 (0.0770) | 0.825 | 80.4 | 61 (19, >10max) | −3.61 (0.1202) | 2.80 (0.0415*) | 0.402 | 79.3 |
| 38.75 | 2.9 (0, >10max) | −3.82 (0.2075) | 11.40 (0.1234) | 0.686 | 80.8 | |||||
| 41.75 | 3.6 (0, >10max) | −2.45 (0.1649) | 6.84 (0.0599) | 0.319 | 83.3 | 34 (19, >10max) | −5.31 (0.0804) | 4.36 (0.0436*) | 0.891 | 83.3 |
| 44.75 | 2.7 (0, >10max) | −3.36 (0.3642) | 10.98 (0.2516) | 0.650 | 75.0 | |||||
| 47.5 | 2.3 (0, >10max) | −32.09 (0.7874) | 89.84 (0.7848) | 0.967 | 80.4 | |||||
| 50.5 | 2.3 (0, >10max) | −38.75 (0.7651) | 108.25 (0.7627) | 0.999 | 80.4 |
Assay reportable values are provided for study time points where a statistically significant correlation was observed in logistic regression models. The survival probability represents survival of a challenge at any of the time points after completing the 3-IM priming series. The intercept represents the log odds of animals with a parameter value of 1 surviving challenge. All values are reported on the original scale of measurements. Values greater than 10 times the maximum value are displayed as “>10max.” *, statistically significant effect (P < 0.05) of the log-adjusted parameter response on survival.
P values of <0.05 indicate a lack of fit. There were no adjustments for multiple testing.