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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Biomaterials. 2012 Aug 29;33(33):8372–8382. doi: 10.1016/j.biomaterials.2012.08.021

Figure 3.

Figure 3

Tight junction protein localization in embryonic day 13 (E13) and adult submandibular salivary glands (SMG). Immunostaining and confocal images show (A) ZO-1 and β-actin in E13 and adult SMG. ZO-1 is distributed around all plasma membranes in epithelial buds with few areas of localization in E13 SMG but is focally concentrated at apical membranes in adult tissue adjacent to apical concentrations of β-actin (arrows). (B) Occludin and aquaporin 5 (Aqp5) localization in E13 and adult SMGs. In E13 SMGs, occludin is detected only in a few focal areas in undifferentiated buds showing no localized Aqp5 (arrows). In adult SMG, occludin is apically concentrated in both ducts (Aqp5 negative, open arrowheads) and in acini (Aqp5 positive, solid arrowheads).