Figure 3. LD bound histones can kill bacteria in vivo.
(A) Schematic representation of embryo microinjection. Early embryos collected within half an hour of laying were injected with a bacterial suspension, as detailed in ‘Materials and methods’. (B). Wild-type and Jabba mutant embryos show similar survival when injected with buffer alone. Wild-type and Jabba mutants (Jabbaf07560, Jabbazl01) embryos were injected with microinjection buffer (no bacteria) and the percentage survival was scored 72 hr post injection. (C) Bacteria grow only in embryos lacking droplet-bound histones. Approximately equal numbers of GFP labeled bacteria (E. coli strain YD133) were injected into wild-type and Jabba mutant embryos (Jabbaf07560) and the growth of bacteria inside embryos was monitored at various times post injection. (D). Drosophila embryos lacking droplet-bound histones have reduced survival due to bacterial infection. Approximately equal numbers of bacteria were injected into wild-type and Jabba mutant (Jabbazl0 and Jabbaf07560) embryos and embryo survival after 72 hr was normalized to the buffer-only injected embryos (in B). The bacterial strains used were Staphylococcus epidermidis (Gram-positive); E. coli DH 5α (Gram- negative); Listeria monocytogenes (Gram-positive and intracellular); and Bacillus subtilis (hlyA), modified Bacillus subtilis expressing listeria hemolysin-A protein (Gram-positive and intracelluar).
Figure 3—figure supplement 1. 2 hr: Additional images of wild-type and Jabba mutant embryos with fluorescent bacteria, 2 hr after bacterial injection.
Figure 3—figure supplement 2. 24 hr: Additional images of wild-type and Jabba mutant embryos with fluorescent bacteria, 24 hr after bacterial injection.
Figure 3—figure supplement 3. 48 hr: Additional images of wild-type and Jabba mutant embryos with fluorescent bacteria, 48 hr after bacterial injection.
