Figure 4. The Jabba protein contributes to improved survival for adult flies.
(A and B) Adult Drosophila lacking Jabba (A: Jabbaf07560; B: Jabbazl01) have reduced survival when challenged by bacteria. Wild-type and Jabba mutant (Jabbazl0 and Jabbaf07560) adult flies were infected with Listeria monocytogenes as detailed in ‘Materials and methods’, and fly survival was monitored over the course of 4 days. (C). Representative plates in a colony forming assay, showing bacterial colonies on agar plates streaked with cytosolic extract from bacteria infected adult flies. (D) Western blots of histone H2B from equal amounts of cytosolic extracts from wild type and Jabba mutant adult flies, showing that overall levels of H2B were significantly reduced in the Jabba mutants.