Figure 5. Bacterial cell wall components release droplet bounds histones in a dose dependent manner.
(A). Increasing concentrations of lipopolysaccharide (LPS) in the buffer releases droplet bound histones from purified LDs. (B). Lipoteichoic acid (LTA) causes the dose dependent release of histones from purified droplets. LDs were purified from wild-type Drosophila embryos, re-suspended in buffer, and incubated for 2 hr at room temperature with different concentrations of LPS or LTA. LDs (LD) were then separated from the under-layer buffer (UB) and both were processed for SDS-PAGE. Western Blot analysis was carried out with H2B histone antibodies.