Figure 1.

Incorporation of UDP-[³H]Glc into Golgi vesicles, and determination of radiolabeled products insoluble in 70% ethanol and 10% TCA. Golgi vesicles (100 μg of protein) were incubated with 1 μm (100,000 cpm) UDP-[³H]Glc for various times. The reaction was stopped by separating the vesicles from the incubation medium by filtration (▪), or by adding ethanol (○) or TCA (•) to a final concentration of 70 or 10%, respectively. The samples were put on ice and filtered, and the radioactivity was determined by liquid-scintillation counting. The total amount of insoluble radioactivity was determined by the summation of the 70% ethanol and 10% TCA-insoluble material (▵). The results are expressed as the percentage of the total radioactivity in UDP-[³H]Glc initially present in the assay. The experiment was repeated three times in duplicate, and the average values and their deviations are plotted. A representative experiment is shown.