Figure 3.

Metabolism of [α-³²P]UDP-Glc and [β-³²P]UDP-Glc in pea stem Golgi vesicles (100 μg of protein) incubated with 1 μm (1 μCi/nmol) of [α-³²P]UDP-Glc or [β-³²P]UDP-Glc. After the indicated times an aliquot equivalent to 2 μg of protein was taken, boiled for 1 min, and loaded on a PEI-TLC plate. The TLC plate was run as described in “Materials and Methods,” air-dried, and exposed to −70°C with an enhancing screen. The migration of standards is shown with arrows. The uridine derivative standard was visualized by UV absorption and the Pi migration standard was visualized using ³²Pi.