FIG. 5.

Effect of sulfur compds 1–4 on LPS-induced activation of mitogen-activated protein kinases in LPS-stimulated RAW 264.7 cells. Cells were pretreated with compds for 30 min prior to LPS stimulation. After treatment with LPS for an additional 15 min, proteins were extracted, and the levels of phosphorylated p38, c-Jun amino-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK) were analyzed by western blot. Images are the representative of three independent experiments that shows similar results. The relative intensity of phospho-p38 (p-p38)/p38, phospho-JNK (p-JNK)/JNK, and phospho-ERK (p-ERK)/ERK bands was measured by densitometry. Data are mean±SD values of three individual experiments. P<.01, significant difference from LPS alone for *p-p38 and ^#p-ERK.