Table 1.
Proteins Identified Following One-Dimensional Gel Electrophoresis
| Peptide sequence | Protein name (Da) | Organism with homologous target | Scorea | Coverage % |
|---|---|---|---|---|
| R.ANTIEAQLK.E | Tropomyosin (33) | Heligmosomoides polygyrus | 262 | 36 |
| R.LEDELVHEK.E | ||||
| K.IVELEEELR.V | ||||
| K.LAMVEADLER.A | ||||
| K.EAQMLAEEADR.K | ||||
| R.MTLLEEELER.A | ||||
| K.VQEAEAEVAALNR.R | ||||
| K.EVDRLEDELVHEK.E | ||||
| K.AQEDLATATSQLEEK.D | ||||
| R.ALQASCLAK.W | Hypothetical protein CBG15316 (39) | Caenorhabditis briggsae | 172 | 9 |
| K.GILAADESTGSMEK.R | ||||
| R.GAAQNIIPAATGAAK.A | Glyceraldehyde-3-phosphate dehydrogenase (36) | Dictyocaulus viviparus | 131 | 10 |
| R.VPTPDVSVVDLTCR.L | ||||
| K.AGFAGDDAPR.A | Actin-2 (41) | Ascaris suum | 134 | 15 |
| K.DSYVGDEAQSK.R | ||||
| K.QEYDESGPSIVHR.K | ||||
| R.VAPEEHPVLLTEAPLNPK.A | ||||
| K.ITETVLSYCYR.A | Aldolase (39) | Haemonchus contortus | 88 | 6 |
| K.KPWALTFSYGR.A | ||||
| K.EPDWVQSER.E | CALUmenin (calcium-binding protein) homolog family member (36) | Caenorhabditis elegans | 87 | 12 |
| R.HLVGIADDNKDGK.L | ||||
| R.DWIMPVGFDHAEAEAR.H | ||||
| R.LLLEQMSQDPGAVR.E | TPR domain (31) | Brugia malayi | 78 | 7 |
| K.LMEFQR.A | ||||
| R.DYGVLKEDDGIAYR.G | Peroxiredoxin (21) | Ascaris suum | 75 | 17 |
| R.LVQAFQFVDK.H | ||||
| R.QITVNDLPVGR.S |
a
Mascot score is −10×log (P), where P is the probability that the observed match is a random event. Mass is molecular weight in kilodaltons.
The band on the area of 31-kDa was excised from the gel and subjected to trypsin digestion, and then analyzed by mass spectrometry for protein identification.