Table 2.

Identification of the 31-kDa Proteins Excised from A. cantonensis Two-Dimensional Gel Electrophoresis Preparations

Spot no.	Peptide sequence	Protein identified (kDa)	Organism with homologous target	Scorea	Coverage (%)
2	K.ADLVNNLGTIAK.S	Heat shock protein 90 (80)	Loa loa	201	7
	K.EDQTEVLEER.R	Heat shock protein 90 (81)	Haemonchus contortus	144	5
	R.ELISNSSDALDK.I
	K.TLTIMDTGIGMTK.A
	R.YQALTEPAELESGK.E
	R.VLSSIEQK.T	14-3-3 product (29)	Meloidogyne incognita	111	14
	K.DSTLIMQLLR.D
	K.SQQSYQEAFDIAK.D
	K.SPGSDTYIVFGEAK.I	NAC domain-containing protein (24)	Brugia malayi	136	12
	K.NILFVINKPDVYK.S
	K.AGIVFTGK.G	PCNA (proliferating cell nuclear antigen) (29)	Caenorhabditis briggsae	98	11
	K.YMNQFTK.A
	K.LEVGLFDTYR.C
3	R.YDDMAQSMK.K	14-3-3 protein isoform 2 (28)	Ancylostoma caninum	234	34
	K.DSTLIMQLLR.D
	R.DICQDVLNLLDK.F
	K.VTELGAELSNEER.N
	K.SQQSYQEAFDIAK.D
	K.MQPTHPIR.L
	K.SPGSDTYIVFGEAK.I	NAC domain containing protein (24)	Brugia malayi	171	12
	K.NILFVINKPDVYK.S
	K.EDQTEVLEER.R	Heat shock protein 90 (80)	Loa loa	128	7
	R.ELISNSSDALDK.I
	K.TLTIMDTGIGMTK.A
	R.YQALTEPAELESGK.E
4	R.LAEYQNATDK.Q	Putative epsilon subunit of coatomer protein complex isoform 2 (33)	Angiostrongylus cantonensis	289	30
	K.ASLVLNEISER.T
	K.AKENLFDELVAA
	K.DAEALLHEAQLR.D
	R.DINPNHPWVIDLK.A
	R.VISSIEQK.T	14-3-3b protein (28)	Meloidogyne incognita	277	29
	K.DSTLIMQLLR.D
	K.VTELGAELSNEER.N
	K.SQQSYQEAFDIAK.D
	R.VGPGIGEYIFDK.E	Putative ferritin protein 2 (6.8)	Angiostrongylus cantonensis	155	67
	K.FLDEQVESIAEIAK.M
	K.ASAANDPHMSDFLESK.F
	K.LAQIISQFER.A	Putative uncoordinated protein 23 (11)	Angiostrongylus cantonensis	150	39
	R.ALTSVNSLIEGVVQK.M
	K.SPGSDTYIVFGEAK.I	NAC domain containing protein (24)	Brugia malayi	92	6

The spots were excised from respective gels and subjected to trypsin digestion and then analyzed by mass spectrometry for protein identification.

^aMascot score is −10×log (P), where P is the probability that the observed match is a random event. Mass is the molecular weight in kilodaltons.