Table 4.
Locally-Bred Horses Seropositive for Antibodies to St. Louis Encephalitis Virus
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HIA titer |
Epitope b-ELISA (%I)a |
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| No. | Sample ID | Locality by town | Gender | Age group (y) | WNV | SLEV | ILHV | FLAV | WNV | SLEV | SLEV PRNT90 titerb | WNV PRNT90 titerc |
| 1 | 1MG4H | Moruga | F | >5 | 0 | 1:40 | 0 | 60.8 | 0 | 31.0 | >640 | <20 |
| 2 | 1MG9H | Moruga | F | 1–5 | 0 | 1:20 | 0 | 31.7 | 0 | 32.0 | 80 | <20 |
| 3 | 1MY7H | Mayaro | F | 1–5 | 0 | 1:40 | 0 | 42.2 | 0 | 20.5 | 160 | <20 |
| 4 | 1OR10H | Oropune | F | >5 | 0 | 1:80 | 0 | 39.1 | 0 | 0 | 160 | <20 |
| 5 | 1OR17H | Oropune | F | >5 | 0 | 1:40 | 0 | 30.2 | 19.7 | 9.6 | 40 | 40 |
| 6 | 1V2H | Valencia | F | >5 | 1:40 | 1:160 | 1:40 | 46.1 | 0 | 37.5 | >640 | 20 |
| 7 | 4RT16H | Arima | M | 1–5 | 0 | 1:20 | 0 | 38.5 | 0 | 19.5 | 80 | 20 |
| 8 | 4RT7H | Arima | F | 1–5 | 0 | 1:20 | 0 | 33.2 | 0 | 36.1 | 40 | <20 |
| 9 | 5RT10H | Arima | M | 1–5 | 0 | 1:20 | 0 | 31.1 | 0 | 15.5 | 40 | <20 |
| 10 | 1GU4H | Guanapo | F | >5 | 0 | 1:40 | 0 | 33.3 | 0 | 13.0 | <20 | <20 |
a
Interpretation for epitope-blocking ELISA: <30% inhibition (%I) is antibody-negative, and >30% inhibition is antibody-positive.
b
Plaque reduction neutralization test (PRNT) for SLEV (titers are expressed as the reciprocal of the serum dilution yielding 90% reduction in the number of plaques).
c
Plaque reduction neutralization test for WNV (titers are expressed as the reciprocal of the serum dilution yielding 90% reduction in the number of plaques).
ELISA, enzyme-linked immunosorbent assay; HIA, hemagglutination inhibition assay; SLEV, St. Louis encephalitis virus; WNV, West Nile virus; ILHV, Ilheus virus.