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. Author manuscript; available in PMC: 2012 Nov 7.
Published in final edited form as: Metabolism. 2011 Jan 19;60(8):1070–1080. doi: 10.1016/j.metabol.2010.11.003

Figure 4. Atherosclerotic lesion area and iron staining in thoracic aortas from control and STZ-DM monkeys.

Figure 4

Cross sections of aorta tissue were fixed for immunohistochemistry and stained with Perl’s stain for ferric iron (Fe3+) as described in Methods. Morphometric analysis was used to determine atherosclerotic lesion area (white bars). Perl’s stain for Fe3+ (black bars) was quantified by assigning a grade of 0, 1, 2, or 3 based on presence or absence of blue staining as well as the number of stained regions within a section.