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. Author manuscript; available in PMC: 2012 Nov 7.
Published in final edited form as: Metabolism. 2011 Jan 19;60(8):1070–1080. doi: 10.1016/j.metabol.2010.11.003

Figure 5. Arterial superoxide levels in control and streptozotocin-treated monkeys.

Figure 5

Segments of carotid and aortic arteries were assessed for superoxide levels as outlined in Methods. Panel A: data are the mean ± SEM for superoxide anion-induced 2-hydroxyethidium fluorescence in aortic segments isolated from control (white bars; n = 9) and STZ-DM monkeys (black bars; n = 9). Arterial rings were incubated in dihydroethidine and subjected to HPLC separation. Formation of 2-hydroxyethidium in the presence of superoxide anion was quantified by fluorescence area-under-the-curve. Panel B: data are the mean ± SEM for the ratio of NADPH-stimulated to basal chemiluminescence in carotid arteries isolated from control (white bars; n = 9) and STZ-DM monkeys (black bars; n = 9). Luminescence is produced by the reaction of lucigenin with superoxide anion in the presence of NADPH. (* = P < 0.05).