Table 3.
Control | STZ-DM | |
---|---|---|
EcSOD | 2.84 ± 0.31 | 2.52 ± 0.15 |
MnSOD | 4.09 ± 0.85 | 4.49 ± 0.90 |
Cu/ZnSOD | 0.41 ± 0.05 | 0.38 ± 0.05 |
Catalase | 0.065 ± 0.011 | 0.067 ± 0.010 |
Heme Oxygenase-1 | 0.028 ± 0.002 | 0.030 ± 0.004 |
Aortic tissue was frozen in liquid nitrogen, and protein fractions containing the SODs, catalase, and heme oxygenase-1 were isolated and subject to gel electrophoresis and immunoblotting as outlined in Methods. Membranes were developed using an ECL-plus detection reagent and quantified by expressing their respective densities as arbitrary units normalized to that of β-actin bands as outlined in Methods. Values are means ± SEM (n = 10 animals per group).