Figure 3. CKD enhances sepsis-induced splenic apoptosis.
CD-1 mice were untreated (normal, A, B) or subjected to 5/6 Nx (C, D) 4 weeks prior to sham (A, C) or CLP surgery (B, D). Representative images of spleen stained for activated caspase3 are shown (A–D); black bar corresponds to 200 μm. Number of activated caspase3 positive cells per high powered field in spleen of composite control at 16 weeks (n=9); C57BL/6 5/6 Nx at 16 weeks (n=4); 129S3 5/6 Nx at 4, 8, 12 weeks (n= 4/group); CD-1 5/6 Nx at 2, 4 weeks (n=4/group)