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. Author manuscript; available in PMC: 2013 Oct 25.
Published in final edited form as: Neuroscience. 2012 Jul 31;223:77–91. doi: 10.1016/j.neuroscience.2012.07.043

Figure 4.

Figure 4

TH-expressing colorectal and urinary bladder DRG neurons colocalize with CGRP. Optical immunofluorescence photomicrographs of sections of T11 (A–D), S1 (E–H), L1 (I–L) or S2 (M–P) DRGs after co-incubation with TH (B, F, J, N) and CGRP antisera (A, E, I, M). Retrogradely labeled colorectal (A–H) or urinary bladder (I–P) neurons containing FB (A, E, I, M) are shown in red. (D, H, L, P) show merged micrographs. (A–P) Several TH-IR NPs are detected (arrowheads in B, F, J, N). Most of the TH-only DRG NPs lack CGRP-LI, with the exception of occasional neurons coexpressing both, the enzyme and the peptide (black double arrowhead in A–D; I–L). Additional non-traced CGRP-IR NPs are also detected (black arrowheads in C, G, K, O). A number of FB+TH+ colorectal (double arrows in E–H) or urinary bladder (double arrows in I–P) NPs are detected, often coexpressing with CGRP. However, some FB+TH+ colorectal (white double arrowhead in A–D) or urinary bladder (white double-arrowhead in M–P) NPs lacking CGRP-LI are also present. Scale bars: 50 µm (D=A-C, M–P; H=E–G; I–L).