Skip to main content
. Author manuscript; available in PMC: 2013 Oct 25.
Published in final edited form as: Neuroscience. 2012 Jul 31;223:77–91. doi: 10.1016/j.neuroscience.2012.07.043

Figure 6.

Figure 6

The main source of autonomic TH fibers in the colorectum and the urinary bladder is the LSC. Optical (A–F; G, J, M) and confocal (H, K, N) immunofluorescence photomicrographs of sections of the LSC (A–F) or MPG (G–O) after incubation with TH (B, E, H, K, N) antiserum. Retrogradely labeled colorectal (A–C; G–I) or urinary bladder (D–F; J–O) neurons containing FB (A, D, G, J, M) are shown in red. (C, F, I, L, O) show merged micrographs. (A–F) A number of FB+TH+ colorectal (A–C) or urinary bladder (D–F) NPs is detected in the LSC (double arrows). In addition, numerous TH-only NPs are also present (arrowheads in B, E). (G–O) In most cases, FB+ colorectal (arrows in G) or urinary bladder (arrows in J, M) NPs lacked TH-LI in the MPG. Likewise, several TH-only NPs could be detected (arrowheads in H, K, N). However, a few TH-IR colorectal (double arrows in G–I) and occasional urinary bladder (double arrows in M–O) NPs are detected. TH-IR fibers were abundant in the MPG (black arrows in H, N). Scale bars: 50 µm (I=A–H; L=J, K; O=M, N).