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. Author manuscript; available in PMC: 2013 Oct 25.
Published in final edited form as: Neuroscience. 2012 Jul 31;223:77–91. doi: 10.1016/j.neuroscience.2012.07.043

Figure 9.

Figure 9

TH and CGRP appear to be present in different populations of nerve fibers innervating the colorectum or the urinary bladder. Confocal immunofluorescence photomicrographs of parasagittal (A–I) and transverse (JR) sections of the colorectum (A–I) or the urinary bladder (J–R), after coincubation with TH (A, D, G, J, M, P) and CGRP (B, E, H, K, N, Q) antisera. (C, F, I, L, O, R) show merged figures. (A–I) In the colorectum, TH- or CGRP-IR fibers were seen in close apposition (A–C). However, closer examination showed that the two markers were present in different nerve populations. This was seen, both in the mucosal layer (inset in C, magnified in D–F), as well as at the level of the myenteric plexus in the muscular layers (G–I). (J–R) In the urinary bladder, and as for the colorectum, TH- (arrowheads in M, P) and CGRP-IR fibers (arrows in N, Q) were found closely juxtaposed with each other (J–L), and virtually always present in different fiber populations. Inset in L is shown at higher magnification in M–L. Scale bars: 20 µm (C=A, B; F=D, E; I= G, H; L=J, K); 10 µm (R=P, Q; O=M, N).