Fig 9.

Localization of AgtA–GFP in a ckiA conditional mutant. Strain VIE050 was grown for 14–16 h in the presence of ammonium (NH4⁺ o/n) or GABA (GABA o/n) as nitrogen sources and then shifted to supplemented MM containing GABA or Urea as nitrogen sources, with or without 10 µM thiamine (+ thi) for the additional time indicated. For details on growth conditions, see Experimental procedures. Arrows indicate structures that are not stained by CMAC. Scale Bars: 5 µm.