Fig 10.

AgtA-3HA protein levels in ckiA mutants. Mycelia of LH121 (ckiA⁺), LH127 (ckiA1919), CAM13 (ckiA102) were pre-grown for 14 h at 30°C on ammonium as sole nitrogen source and transferred to either ammonium (NH₄⁺) or γ-aminobutyric acid (GABA) for an additional 3 h incubation at 37°C. The ckiA allele used is indicated above each track. Loading was monitored by Coomassie blue staining and membranes were reacted with anti-HA primary antibodies and stained as described in Experimental procedures. Northern blots (not shown) carried out in parallel showed an identical pattern of de-repression of agtA after transfer to GABA (Apostolaki et al., 2009) for wild type and mutant strains. In a separate analogous experiment LH121 (ckiA⁺) was compared with AMC264 (ckiA2). The latter behaved exactly as ckiA102 (not shown).