Table 2.

Summary of time-resolved tryptophan fluorescence measurements of Pdc(W29)

Sample	τmean∗† (ns)	β1∗∗	ϕ1|| (ns)	β2∗∗	ϕ2|| (ns)	β3∗∗	ϕ3|| (ns)	RH‡ (Å)
Pdc	3.9	0.045	0.5	0.094	2.4	0.061	36	34
Pdc + 14-3-3§	3.9	0.051	0.5	0.088	2.4	0.062	61	41
dpPdc	3.8	0.057	0.4	0.102	2.7	0.043	36	34
dpPdc + 14-3-3	4.2	0.028	0.4	0.067	1.8	0.108	71	43

^∗SD > 0.05 ns.

^†The mean fluorescent lifetime (τ_mean) was calculated using Eq. 2.

^‡The hydrodynamic radius R_H was estimated from the equation ${\varphi }_3=\eta V/kT$, where η is water viscosity, T is temperature, k is the Boltzmann constant, and V is volume of the spherical rigid rotor $\left(V=4/3\pi R_H^3\right)$.

^§The human 14-3-3noW protein mutant missing all Trp residues (mutations Trp59Phe and Trp228Phe) was used in all Pdc tryptophan measurements (27,28,36).

^||SD < 20%.

^∗∗SD < 0.01.