. 2012 Nov 7;103(9):1960–1969. doi: 10.1016/j.bpj.2012.09.021

Table 3.

Summary of time-resolved fluorescence measurements of AEDANS-labeled single-cys mutants of Pdc

Pdc mutant			τ_mean^∗^† (ns)	β₁^§	ϕ₁^‡ (ns)	β₂^§	ϕ₂^‡ (ns)	β₃^§	ϕ₃^‡ (ns)	β₄^§	ϕ₄ (ns)
Cys-89	Not-P		15.00	0.027	0.4	0.093	2.5	0.078	13	0.032	> 50
	Not-P	+ 14-3-3	15.01	0.044	0.5	0.093	3.0	0.068	16	0.025	> 50
	P		14.67	0.029	0.4	0.092	2.1	0.066	8	0.043	> 50
	P	+ 14-3-3	15.52	0.013	0.3	0.076	2.1	0.066	9	0.075	> 50
Cys-97	Not-P		15.28	0.054	0.5	0.083	3.3	0.056	12	0.037	> 50
	Not-P	+ 14-3-3	15.28	0.035	0.4	0.090	2.5	0.059	11	0.046	> 50
	P		14.73	0.052	0.5	0.096	2.5	0.066	15	0.016	> 50
	P	+ 14-3-3	15.33	0.052	0.5	0.076	3.3	0.055	15	0.047	> 50
Cys-148	Not-P		15.70	0.006	0.3	0.088	2.7	0.071	12	0.065	> 50
	Not-P	+ 14-3-3	15.51	0.022	0.5	0.077	3.0	0.074	12	0.057	> 50
	P		14.99	0.023	0.4	0.078	2.5	0.084	11	0.045	> 50
	P	+ 14-3-3	15.14	0.011	0.3	0.075	2.1	0.083	10	0.061	> 50
Cys-157	Not-P		14.70	0.023	0.5	0.094	3.0	0.096	18	0.017	> 50
	Not-P	+ 14-3-3	14.69	0.034	0.7	0.080	2.7	0.100	19	0.016	> 50
	P		14.13	0.045	0.6	0.100	3.3	0.071	18	0.014	> 50
	P	+ 14-3-3	14.32	0.038	0.4	0.124	3.0	0.034	23	0.034	> 50
Cys-168	Not-P		15.43	0.032	0.6	0.109	2.7	0.079	25	0.010	> 50
	Not-P	+ 14-3-3	15.42	0.029	0.5	0.099	2.7	0.087	18	0.015	> 50
	P		14.92	0.047	0.5	0.098	2.7	0.075	15	0.010	> 50
	P	+ 14-3-3	15.44	0.022	0.3	0.096	2.7	0.071	18	0.041	> 50

^∗

SD > 0.05 ns.

^†

The mean fluorescent lifetime (τ_mean) was calculated using Eq. 2.

^‡

SD < 20%.

^§

SD < 0.01.