Figure 1.

Complementation of yeast hap2 with AtHAP2 cDNAs. S. cerevisiae strain JO1-1a (MY69) was transformed with three independent Arabidopsis HAP2 cDNAs (P1, P3, and P5 for AtHAP2a, AtHAP2b, and AtHAP2c, respectively) in the yeast expression vector pFL61. After primary selection on medium without uracil, two different dilutions of transformants were plated onto lactate medium along with Δhap2 itself (MY69) and the parental strain with a wild-type HAP2 gene (MY68). A reduced level of complementation was observed for AtHAP2b (P3).