Figure 2.

Accumulation and rarefaction curves of csp variants. A, Variant accumulation and rarefaction curves for the entire population. The thick solid line shows the raw data for the variant accumulation curve. The computed rarefaction curve (thin solid line) represents the expected average rate of variant accumulation that would be produced by repeated deep sequencing of the same population. Confidence intervals (CIs) for the species richness were also determined (dashed and dotted lines represent upper and lower 95% CIs, respectively). CIs for rarefaction curves allow for the comparison of diversity between populations, even when there are differences in sampling effort. B and C, Rarefaction curve (thin solid line) and 95% CIs (dotted lines) for the adult (B) and child (C) populations.