Skip to main content
. 2012 Jan 10;22(12):2878–2893. doi: 10.1093/cercor/bhr365

Figure 5.

Figure 5.

In utero electroporation of lacZ reporter gene constructs into the telencephalon. Shown are GFP immunofluorescences (A,C,E,G,I,K) to detect electroporated cells and X-Gal staining to reveal enhancer activity (B,D,F,H,J,L). (A–D) Electroporation of a Gli3/lacZ reporter gene construct results in reporter gene activation in cortical progenitor cells (A,B), while enhancer activity is abolished by mutations in the Tcf/Lef-binding site (C,D). (E–H) The Nfia telencephalic enhancer is active in the cortical hem and in roof plate (rp) cells (E,F). X-Gal staining is confined to roof plate cells (arrow) after mutating the Tcf/Lef-binding site (G,H). (I–L) Embryo electroporated with a Wnt8b/LacZ reporter gene construct shows X-Gal staining in the hippocampus (hip) (I,J). The Tcf/Lef mutant Wnt8b/LacZ reporter construct lacks enhancer activity in the dorsomedial telencephalon (K,L).