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. 1998 Jul;117(3):1023–1030. doi: 10.1104/pp.117.3.1023

Figure 6.

Figure 6

Effects of buffer alone and water-soluble cAMP and cGMP on [Ca2+]cyt levels in tobacco protoplasts. Media for cAMP, cGMP, and buffer plus ethanol (200-μL solutions) were the same as for Figure 1. Peak [Ca2+]cyt values after the addition of cyclic mononucleotides and Ca2+ to protoplasts were calculated after chemiluminescence discharge under the action of 0.1% Triton X-100/100 mm CaCl2 as described previously (Cobbold and Rink, 1987; Knight and Knight, 1995).