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. 2012 Oct 2;4(10):1112–1125. doi: 10.1002/emmm.201201272

Figure 5. Macrophage accumulation and M1 inflammatory factor expression in VAT from DIO mice treated with or without different ODNs or in vitro cultured macrophages.

Figure 5

  • A. Immunostaining of VAT with rat anti-mouse Mac-3 mAb. Graph represents the number of Mac-3+ cells in each 10 fields.
  • B-E. RT-PCR determined mRNA levels of (B) M1 markers (TNF-α, IL-6, IP-10 and MCP-1) and (C) M2 markers (Arg1, Mrc1 and IL-10) in VAT after normalizing to GAPDH. Fold change was calculated relative to PBS group. RT-PCR determined mRNA levels (normalized to GAPDH) of M1 markers (TNF-α, IL-6, IP-10 and MCP-1) and M2 markers (Arg1, Mrc1 and IL-10) in untreated BMDMs (D) or BMDMs that were stimulated with LPS (10 ng/ml) and IFN-γ (100 U/ml) for 24 h (E), with or without 5 µM ODNR01 or ODN1612. Fold change was calculated relative to medium group (−). Data represent three independent experiments. *p < 0.05; **p < 0.01, n = 4∼6.