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. Author manuscript; available in PMC: 2012 Nov 7.
Published in final edited form as: J Biol Chem. 2004 Apr 21;279(28):29493–29500. doi: 10.1074/jbc.M403187200

Figure 3. SDS-PAGE of trypanothione reductase inactivated by quinacrine mustard.

Figure 3

TryR (0.1 mg ml−1) was incubated with or without NADPH (150 μM) and QM (20 μM) for 10 min and the reaction quenched by the addition of excess 2-mercaptoethanol (25 mM). Samples were separated by 10% SDS PAGE and examined under UV-illumination followed by staining with Coomassie blue. Panel A: Coomassie stained gel: lane 1, untreated control; lane 2, plus QM without NADPH; lane 3; plus QM with NADPH. The arrow heads point to an additional faint band at 110 kDa. Panel B: UV-illumination, lanes as in panel A.