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. Author manuscript; available in PMC: 2013 Oct 24.
Published in final edited form as: J Am Chem Soc. 2012 Oct 11;134(42):17643–17652. doi: 10.1021/ja307102g

Figure 9.

Figure 9

% Expression of the Renilla luciferase relative to the firefly luciferase, when treated with siRNAs bearing modifications at position 6 in the guide strand, and targeted U (from dA6:dT plasmid, depicted by solid bars) vs. G (from dC6:dG plasmid, depicted by hashed bars) in the caspase 2 insert and mutant caspase 2 insert mRNA respectively. Sequences are given in Figure 2. Experiments were conducted at two different concentrations: 50 nM and 0.5 nM. A:U, Pg6:U, Ce6:U and Pe6:U represents targeting of U in the caspase 2 insert mRNA (from dA6:dT plasmid) and C:G, A:G, Pg6:G, Ce6:G and Pe6:G represents targeting of G in the mutant mRNA where a U was mutated to G (from dC6:dG plasmid). Nomenclature of the plasmid comes from the corresponding modification position in the guide strand of caspase 2 insert mRNA.