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. 2012 Jan 18;32(3):880–889. doi: 10.1523/JNEUROSCI.3707-11.2012

Figure 2.

Figure 2.

UAS transgenes can be selectively targeted to the BSEG and BAG neurons. A, B, UAS transgene expression (green, UAS-EGFP) can be targeted to the two subsets of bursicon-expressing neurons (magenta, anti-burs) using the BSEG-specific (i.e., ETVP16AD-99 ∩ Burs-Gal4DBD-U6A1; A) or the BAG-specific (i.e., ETVP16AD-N9A88A ∩ Burs-Gal4DBD-U6A1; B) hemidriver combinations. Images are volume-rendered fluorescent confocal micrographs of CNS whole mounts. Scale bars, 50 μm. A′, B′, The corresponding patterns of anti-bursicon α-subunit labeling for the preparations shown in A and B, respectively. A″, B″, Merged images of the patterns of EGFP and anti-bursicon labeling for the two preparations.