Figure 5.

B_AG suppression blocks somatic, but not behavioral, aspects of wing expansion. A, Video frame of a B_AG>Kir2.1 fly showing that the execution of abdominal flexion (arrow) and proboscis extension required for air swallowing (arrowhead) are normal. B, Both B_AG>Kir2.1 (left) and B_AG>pburs-RNAi (right) flies swallow substantial amounts of air during the period of sustained abdominal flexion relative to matched controls (i.e., either flies lacking the DBD hemidriver or the UAS-pburs-RNAi transgene, respectively). , males; ♀, females. C, Bar graphs showing the median time from eclosion to the onset of the first bout sustained abdominal flexion (Delay) and the average duration of the first bout (Bout 1) in B_AG>pburs-RNAi flies (n = 7) versus matched controls (Con) lacking the UAS-pburs-RNAi transgene (n = 9). Error bars are SD. D, Wing expansion deficits observed in B_AG>Kir2.1 flies in noninhibitory (−) and inhibitory (+) environments are similar (left) and somewhat less severe than those observed in B_AG>pburs-RNAi flies (right). EW, Expanded wings, UW, unexpanded wings; PW, partially expanded wings. E, Left and middle, Wing expansion (arrow) and cuticle tanning (arrowhead) are impaired in B_AG>Kir2.1 animals (left; note reduced pigmentation), relative to controls lacking the B_AG hemidriver (middle). Right, the wings (arrow) of B_AG>pburs-RNAi flies typically undergo little to no expansion during abdominal flexion.