Table 1.
The loading capacity and extraction recovery of single-StageTip microcolumns
| |
Recovery of3 H-labelled CKs (%) |
|||||
|---|---|---|---|---|---|---|
| |
C18 |
SDB-RPS |
Cation-SR |
|||
| Flow through | Elution | Flow through | Elution | Flow through | Elution | |
| [3H]cZ |
98.8 ± 3.3 |
0.6 ± 0.2 |
1.3 ± 0.1 |
96.6 ± 1.8 |
1.1 ± 0.1 |
100.7 ± 3.0 |
| [3H]tZR |
98.8 ± 4.4 |
0.6 ± 0.2 |
39.2 ± 2.2 |
29.8 ± 2.2 |
22.4 ± 0.6 |
64.7 ± 1.1 |
| [3H]iPR | 102.9 ± 3.5 | 0.5 ± 0.3 | 4.9 ± 0.2 | 95.6 ± 1.4 | 4.7 ± 0.1 | 97.0 ± 2.8 |
Values are means ± SD (n = 4).
Recovery (%) of 3 H-labelled CK standards without added plant matrix. Tritium-labelled standards (105 dpm) were dissolved in Bieleski buffer and applied onto different Empore sorbents (C18, SDB-RPS, Cation-SR).