Table 2.

Method parameters

Compounds	Precursor	Products	Retention timea(min)	LODb(fmol)	Dynamic range (mol)	R2
t/cZ	220.1	136.1, 119.0	15.39 ± 0.04/16.82 ± 0.03	0.5	1×10−15–5×10−11	0.9989/0.9987
t/cZR	352.2	220.1, 136.1	19.06 ± 0.02/19.72 ± 0.01	0.1	5×10−16–5×10−11	0.9993/0.9986
tZ7G	382.2	220.1, 136.1	12.26 ± 0.02	0.1	5×10−16–1×10−11	0.9989
t/cZ9G			14.23 ± 0.02/15.13 ± 0.02	0.1	5×10−16–5×10−11	0.9993/0.9985
t/cZOG			14.83 ± 0.03/15.79 ± 0.03	0.5	1×10−16–1×10−11	0.9987/0.9988
t/cZROG	432.2	382.2, 220.1	18.08 ± 0.02/18.77 ± 0.02	1.0	5×10−15–1×10−11	0.9992/0.9984
t/cZMP	514.2	220.1, 136.1	13.72 ± 0.02/14.67 ± 0.02	5.0	1×10−14–5×10−11	0.9990/0.9985
DHZ	222.1	136.1, 119.0	16.15 ± 0.04	0.1	5×10−16–1×10−11	0.9991
DHZR	354.2	222.1, 136.1	19.61 ± 0.01	0.05	1×10−16–5×10−10	0.9989
DHZ7G	384.2	222.1, 136.1	13.80 ± 0.02/14.13 ± 0.02	0.1	5×10−16–1×10−10	0.9994
DHZ9G			15.00 ± 0.01	0.05	1×10−16–1×10−10	0.9992
DHZOG			16.37 ± 0.03	0.1	5×10−16–5×10−12	0.9992
DHZROG	434.2	384.2, 222.1	19.22 ± 0.03	1.0	5×10−15–1×10−11	0.9983
DHZMP	516.2	222.1, 136.1	14.34 ± 0.01	1.0	5×10−15–1×10−11	0.9992
iP	204.1	136.1, 119.0	23.21 ± 0.01	0.1	5×10−16–1×10−11	0.9991
iPR	336.2	204.1, 136.1	23.88 ± 0.01	0.05	1×10−16–1×10−11	0.9989
iP7G	366.2	204.1, 136.1	18.70 ± 0.01	0.05	1×10−16–1×10−11	0.9988
iP9G			21.50 ± 0.01	0.5	1×10−15–1×10−11	0.9992
iPMP	416.2	204.1, 136.1	21.31 ± 0.02	5.0	1×10−14–5×10−11	0.9993

^a Values are means ± SD (n = 10); ^b Limit of detection, defined as a signal-to-noise ratio of 3:1.

List of precursor and product ions of the studied compounds (the most abundant product ion is shown in bold type). The retention time stability, limits of detection, dynamic linear range, linearity (coefficient of determination, R²) are shown for UHPLC-ESI(+)-MS/MS analysis of isoprenoid cytokinins.