Table 4.
Recovery (%) of different CK groups in relation to number of sorbent multi-layers (C18, SDB-RPS, Cation-SR) used in PT-SPE purification procedure
| Recovery (%)a | |||
|---|---|---|---|
|
CKs |
Number of sorbent multi-layers a |
||
| |
1 |
2 |
3 |
| Free Bases |
23 ± 3 |
58 ± 13 |
73 ± 15 |
| Ribosides |
11 ± 4 |
33 ± 9 |
77 ± 18 |
|
N-glucosides |
7 ± 1 |
36 ± 23 |
85 ± 5 |
|
O-glucosides |
7 ± 3 |
31 ± 21 |
81 ± 10 |
| Nucleotides | 5 ± 3 | 13 ± 2 | 81 ± 6 |
a Values are means ± SD (n = 4).
Plant tissues (5 mg FW of A. thaliana seedlings spiked with 1 pmol of authentic CK standards) were extracted in Bieleski buffer, purified by StageTip protocol and directly analyzed by UHPLC-ESI(+)-MS/MS.